Product Description

Pan Methylated Lysine Monoclonal Antibody (Mix)

Sizes: 50μL, 100μL

Catalogue Numbers: RA10680-50, RA10680-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Methylation of lysine residues is a common regulatory posttranslational modification (PTM) that results in the mono-, di-, or tri-methylation of lysine at ε-amine groups by protein lysine methyltransferases (PKMTs). Two PKMT groups are recognized based on structure and catalytic mechanism: class I methyltransferases or seven β strand enzymes, and SET domain-containing class V methyltransferases. Both use the methyl donor S-adenosyl-L-methionine to methylate histone and non-histone proteins. Class I methyltransferases methylate amino acids, DNA, and RNA. Six methyl-lysine-interacting protein families are distinguished based on binding domains: mBT, PHD finger, Tudor, PWWP, WD40 repeat, and chromodomains. Many of these display differential binding preferences based on lysine methylation state. KDM1 subfamily lysine demethylases catalyze demethylation of mono- and di-methyl lysines, while 2-oxoglutarate-dependent JmjC (KDM2-7) subfamily enzymes also modify tri-methyl lysine residues.

Reactivity: Species independent

Host: Mouse

Applications: WB, IHC-p, IF(paraffin section)

Clonality: Monoclonal

Dilution: WB: 1:1000-2000 IHC: 1:200-500

Storage: -20°C/1 year

Condition: PBS, pH 7.4, containing 0.5% BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

Research Use Only