Product Description

Pan Mono-Methyl Lysine Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS74114-50, BS74114-100

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IP, ChIP

All Applications: WB,1:500 - 1:2000 | IP,1:50 - 1:200 | ChIP,1:50 - 1:200

Background: Methylation of lysine residues is a common regulatory post-translational modification (PTM) that results in the mono-, di-, or tri-methylation of lysine at ε-amine groups by protein lysine methyltransferases (PKMTs).

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Methylated

Extra Notes: Western blot analysis of extracts of various cell lines, using Pan Mono-Methyl lysine Rabbit pAb at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s., Western blot analysis of extracts of various cell lines, using Pan Mono-Methyl lysine Rabbit pAb at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 3min., Western blot analysis of extracts of various cell lines, using Pan Mono-Methyl lysine antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s., Immunoprecipitation analysis of 1000ug extracts of HeLa cells using 20ug Pan Mono-Methyl lysine antibody. Western blot was performed from the immunoprecipitate using MonoMethyl-Histone H3-K9 at a dilution of 1:1000.

Bioworld Molecular Weight: 17-90kDa

Note: For research use only, not for use in diagnostic procedure.

Immunogen: A synthetic peptide corresponding to a sequence containing monomethylatedK.

Conjugate: Unconjugated

Modification: Methylated