Pan Phospho-Serine/Threonine Rabbit pAb
Sizes: 20μL, 100μL
Catalogue Numbers: AP0893-20, AP0893-100
Citations, Manuals and MSDS Available upon request.
Background: As a critical post-translational modification, phosphorylation plays important roles in regulating various biological processes, Serine/threonine phosphorylation is an important mechanism that is involved in the regulation of protein function. Protein phosphorylation is the most well-studied post translational modification (PTM), in which a phosphoryl group from adenosine triphosphate (ATP) is covalently attached to a serine (~86%), threonine (~12%), or tyrosine (~2%) by a kinase and removed by a phosphatase. Phosphorylation at other amino acids have also been reported. Phosphorylation can modify protein structure, function, and interactions. As such, phosphorylation plays a critical role in virtually all cellular processes in homeostasis and disease, including signal transduction, cell cycle, differentiation, proliferation, metabolism, motility, and death. Importantly, phosphorylation at different residues can cause different outcomes. For example, RAF1 is a kinase central to the MAPK pathway that is activated when it is phosphorylated at serine (S) or threonine (T) residues S259, S338, S340/341, T491, or S494. However, phosphorylation at S289/296/301 results in the inhibition of RAF1 kinase activity.
Applications: Phosphorylated Antibodies
Applications: WB, ELISA
Cross-reactivity: Human, Mouse, Rat, Other (Wide Range Predicted)
Observed Molecular Weight: >10kDa
Immunogen: Synthetic peptide
Source: Rabbit
Isotype: IgG
Purity: Affinity purification
Storage: Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH 7.3.
Recommended Dilutions: WB, 1:500 - 1:1000 ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Research Areas: Protein phosphorylation, Protein phosphorylation, Protein phosphorylation.
NCBI Alias: pan p-S/T
Research Use Only