PAR-2 Polyclonal Antibody Sizes: 50μl, 100μl Catalogue Numbers: BS65550-50, BS65550-100 Lead times: 1-2 weeks, if manufacturer has product in stock Manufacturer/Ship Location: China Background: The Proteinase-activated receptor 2 (PAR2) is a member of the proteinase-activated receptor subfamily. It is activated through proteolytic exposure of an occμlt tethered ligand by trypsin and trypsin-like proteases. This is in contrast to other members of the subfamily which are activated by the protease thrombin. PAR2 has been implicated in acute inflammatory response, asthma, and pain transmission. PAR2 expression has been documented in the periphery. ESTs have been isolated from adrenal, brain, breast, heart/melanocyte/uterus, kidney, lung, and vessel libraries. Coagμlation factor II (thrombin) receptor-like 1 (F2RL1)is a member of the large family of 7-transmembrane-region receptors that couple to guanosine-nucleotide-binding proteins. F2RL1 is also a member of the protease-activated receptor family. It is activated by trypsin, but not by thrombin. It is activated by proteolytic cleavage of its extracellμlar amino terminus. The new amino terminus functions as a tethered ligand and activates the receptor. The F2RL1 gene contains two exons and is widely expressed in human tissues. The predicted protein sequence is 83% identical to the mouse receptor sequence. [provided by RefSeq] Category: Primary Antibody Reactivity: Human Host: Rabbit Applications: IHC-P Alternate Names: Paraformaldehyde-fixed, paraffin embedded (Human kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30min, Antibody incubation with (PAR-2) Polyclonal Antibody, Unconjugated (bs-22639R) at 1:400 overnight at 4°C Clonality: Polyclonal Conjugate: Unconjugated Modification: Unmodification Immunogen: KLH conjugated synthetic peptide derived from human PAR-2:37-100/397 Dilution: IHC-P=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations shoμld be determined by the end user. Purification: affinity purified by Protein A Specificity: PAR-2 polyclonal Antibody detects endogenous levels of PAR-2 protein. Molecular Weight: ~40kDa Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. SwissProt: P55085 Product: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Research Use Only