Product Description

PAR4 (D313) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS1300-50, BS1300-100

Sizes: 50µl, 100µl

Swiss-Prot: Q96IZ0

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

Background: Aberrant regulation of any of these cell processes can result in cancer. Cell death during embryogenesis, tissue atrophy and normal tissue turnover is called apoptosis and is characterized by cytoplasmic and nuclear condensation, nuclear disorganization and fragmentation of genomic DNA into 180-200 base pair oligomers. Five ionomycin-inducible complementary cDNAs, designated PAR1, 2, 3, 4 and 5, have been isolated from the prostate cancer cell line AT-3. Nucleotide sequencing identified PAR1 as the rat homolog of MKP-1, PAR2 as the injury-inducible gene HB-EGF, and PAR3 as the serum-induced gene Cyr61. PAR4 and PAR5 sequences were not found to correspond to any previously described proteins. PAR4 (prostate apoptosis response 4) is specifically expressed by cells entering apoptosis and is not induced during growth factor stimulation, oxidative stress, necrosis or growth arrest. The PAR4 gene encodes a protein with a putative nuclear localization signal and carboxy terminal leucine zipper.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Prostate Apoptosis Response protein-4 (D313) polyclonal antibody detects endogenous levels of Prostate Apoptosis Response protein-4 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of PAR4 (D313) polyclonal antibody at 1:500 dillution
Lane 1: U-87MG whole cell lysate (40μg)
Lane 2: HCT-116 whole cell lysate (40μg)
Lane 3: 786-O whole cell lysate (40μg)
Lane 4: The Testis tissue lysate of Mouse (40μg)
Lane 5: The Testis tissue lysate of Rat (40μg), Immunohistochemistry (IHC) analyzes of PAR4 (D313) pAb in paraffin-embedded human lung carcinoma tissue.

Bioworld Molecular Weight: ~ 45 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: PRKC apoptosis WT1 regulator protein; Prostate apoptosis response 4 protein; Par-4; PAWR

Immunogen: Synthetic peptide, corresponding to amino acids 283-332 of Human PAR4.

Conjugate: Unconjugated

Modification: Unmodified