Product Description

PARD3 (F1161) Polyclonal Antibody

Catalogue Numbers: BS2104-50, BS2104-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q8TEW0

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: Cellular asymmetry is critical for the development of multicellular organisms. PARD (partitioning-defective) proteins play important roles in asymmetric cell division and polarized growth. PARD3A (partitioning-defective 3), also known as Baz, ASIP (atypical PKC isotype-specific-interacting protein), PAR3, PARD3, PAR3α, Bazooka, SE2-5T2, SE2-5L16 or SE2-5LT1, is a 1,356 amino acid protein that contains three PDZ domains and belongs to the PAR3 family of proteins. Expressed in a wide variety of tissues, PARD3A colocalizes with PARD6A/B and PKC ι at epithelial tight junctions and is believed to function as an adapter protein with an important role in the formation of normal tight junctions at epithelial cell-cell contacts. Due to alternative splicing events, PARD3A exists in at least ten isoforms, namely isoform A, isoform B, isoform C, isoform D, isoform E, isoform F, isoform Lb, isoform Sa, isoform Sb and isoform 10.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: PARD3 (F1161) polyclonal antibody detects endogenous levels of PARD3 protein.

Extra Notes: Western blot (WB) analysis of PARD3 (F1161) polyclonal antibody at 1:500 dilution
Lane1:CT26 whole cell lysate(40ug)
Lane2:PC12 whole cell lysate(40ug)
Lane3:HEK293T whole cell lysate(40ug)
Lane4:Hela whole cell lysate(40ug)
Lane5:H1792 whole cell lysate(40ug)

Bioworld Molecular Weight: ~ 181 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: Partitioning defective 3 homolog; PAR-3; PARD-3; Atypical PKC isotype-specific-interacting protein; ASIP; CTCL tumor antigen se2-5; PAR3-alpha; PARD3; PAR3; PAR3A

Immunogen: Synthetic peptide, corresponding to amino acids 1131-1191 of Human PARD3.

Conjugate: Unconjugated

Modification: Unmodified