PARD6A Polyclonal Antibody-BS7481

PARD6A Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS7481-50, BS7481-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9NPB6

Host: Rabbit

Reactivity: Rat

Applications: WB

All Applications: WB,1:500 - 1:2000

Background: This gene is a member of the PAR6 family and encodes a protein with a PSD95/Discs-large/ZO1 (PDZ) domain and a semi-Cdc42/Rac interactive binding (CRIB) domain. This cell membrane protein is involved in asymmetrical cell division and cell polarization processes as a member of a multi-protein complex. The protein also has a role in the epithelial-to-mesenchymal transition (EMT) that characterizes the invasive phenotype associated with metastatic carcinomas. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Polyclonal Antibodies

Bioworld Molecular Weight: 37kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western Blot analysis of extracts of rat skeletal muscle, using PARD6A antibody at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 15s.

Alternative Name: PARD6A; PAR-6A; PAR6; PAR6C; PAR6alpha; TAX40; TIP-40

Immunogen: Recombinant fusion protein of human PARD6A(NP_001032358.1).

Conjugate: Unconjugated

Modification: Unmodified