PARP1 Monoclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: MB9403-50, MB9403-100
Product: Mouse IgG1, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P09874(Human) P11103(Mouse) P27008(Rat)
Host: Mouse
Reactivity: Human, Mouse, Rat
Applications: WB, ICC IHC FC
All Applications: WB:1:500
ICC:1:50-1:100
IHC:1:100-1:500
FC:1:50-1:100
Background: Poly(ADP-ribose) polymerase-1 (PARP-1), also designated PARP, is a nuclear DNA-binding zinc finger protein that influences DNA repair, DNA replication, modulation of chromatin structure, and apoptosis. In response to genotoxic stress, PARP-1 catalyzes the transfer of ADP-ribose units from NAD(+) to a number of acceptor molecules including chromatin. PARP-1 recognizes DNA strand interruptions and can complex with RNA and negatively regulate transcription. Actinomycin D- and etoposide-dependent induction of caspases mediates cleavage of PARP-1 into a p89 fragment that traverses into the cytoplasm. Apoptosis-inducing factor (AIF) translocation from the mitochondria to the nucleus is PARP-1-dependent and is necessary for PARP-1-dependent cell death. PARP-1 deficiencies lead to chromosomal instability due to higher frequencies of chromosome fusions and aneuploidy, suggesting that poly(ADP-ribosyl)ation contributes to the efficient maintenance of genome integrity.
Purification and Purity: Peptide affinity purified
Storage and Stability: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Specificity: PARP1 monoclonal antibody detects endogenous levels of PARP1 protein.
Bioworld Molecular Weight: 113 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of PARP1 on different lysates using anti-PARP1 antibody at 1/100 dilution.
Positive control:
Lane 1: Daudi
Lane 2: Rat spleen tissue, ICC staining PARP1 (green) in 293T cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: PARP-1, ARTD1, ADPRT 1, PARP1, ADPRT, PPOL, ADP ribosyltransferase (NAD+; poly (ADP ribose) polymerase), ADP ribosyltransferase, ADP ribosyltransferase diphtheria toxin like 1, ADP ribosyltransferase NAD(+), ADPRT 1, ADPRT, ADPRT1, ARTD1, msPARP, NAD(+) ADP ribosyltransferase 1, NAD(+) ADP-ribosyltransferase 1, pADPRT 1, pADPRT1, PARP 1, PARP, PARP-1, PARP1, PARP1, Poly (ADP ribose) polymerase 1, poly (ADP ribose) polymerase family, member 1, Poly [ADP-ribose] polymerase 1, Poly(ADP ribose) polymerase, poly(ADP ribose) synthetase, poly(ADP ribosyl)transferase, Poly[ADP ribose] synthetase 1, Poly[ADP-ribose] synthase 1, PPOL,
Immunogen: Peptide
Conjugate: Unconjugated
Modification: Unmodified