Product Description

PARP2 Polyclonal Antibody

Catalogue Numbers: BS67227-50, BS67227-100

Sizes: 50µl, 100µl

Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Swiss-Prot: Q9UGN5

Host: Rabbit

Reactivity: Human, Rat

Applications: WB

All Applications: WB (1/500 - 1/1000)

Background: Poly-ADP-ribosyltransferase that mediates poly-ADP-ribosylation of proteins and plays a key role in DNA repair (PubMed:10364231, PubMed:25043379, PubMed:27471034, PubMed:32028527, PubMed:32939087).
Mediates glutamate, aspartate or serine ADP-ribosylation of proteins: the ADP-D-ribosyl group of NAD+ is transferred to the acceptor carboxyl group of target residues and further ADP-ribosyl groups are transferred to the 2'-position of the terminal adenosine moiety, building up a polymer with an average chain length of 20-30 units (PubMed:25043379, PubMed:30321391).
Serine ADP-ribosylation of proteins constitutes the primary form of ADP-ribosylation of proteins in response to DNA damage (PubMed:32939087).
Mediates glutamate and aspartate ADP-ribosylation of target proteins in absence of HPF1 (PubMed:25043379).
Following interaction with HPF1, catalyzes serine ADP-ribosylation of target proteins; HPF1 conferring serine specificity by completing the PARP2 active site (PubMed:28190768, PubMed:32028527).

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 188% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Recognizes endogenous levels of PARP2 protein.

Extra Notes: Western blot analysis of PARP2 expression in HEK293T (A), MCF7 (B), rat brain (C) whole cell lysates.

Bioworld Molecular Weight: ~ 66 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: ADPRT2; ADPRTL2; Poly [ADP-ribose] polymerase 2; PARP-2; hPARP-2; ADP-ribosyltransferase diphtheria toxin-like 2; ARTD2; NAD(+) ADP-ribosyltransferase 2; ADPRT-2; Poly[ADP-ribose] synthase 2; pADPRT-2

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human PARP2. The exact sequence is proprietary.

Conjugate: Unconjugated

Modification: Unmodified