Product Description

Pax-5 (D2) Polyclonal Antibody

Catalogue Numbers: BS1287-50, BS1287-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q02548

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: The Pax gene family of nuclear transcription factors is comprised of nine family members that function during embryogenesis to regulate the temporal and position-dependent differentiation of cells. In addition, the family is involved in a variety of signal transduction pathways in the adult organism. Mutations in the Pax family of proteins have been linked to disease and cancer in humans. For example, the human Pax-5 gene encodes a B cell lineage specific protein called B cell specific activator protein or BSAP, which is expressed in pro-B, pre-B and mature B lymphocytes but not in plasma cells. BSAP functions to regulate not only B cell development, but also influences the balance between immunoglobulin secretion and B cell proliferation. Overexpression of BSAP has been implicated in cellular transformation, and in the case of small lyphocytic lymphomas with plasmacytoid differentiation, a t(9;14)(p13;q32) translocation resulting in the deregulation of Pax-5 gene expression has been detected. The gene which encodes Pax-5 maps to human chromosome 9p13.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Pax-5 (D2) polyclonal antibody detects endogenous levels of Pax-5 protein.

Extra Notes: Western blot (WB) analysis of Pax-5 (D2) pAb at 1:500 dilution
Lane1:THP-1 whole cell lysate(40ug)
Lane2:K562 whole cell lysate(40ug)
Lane3:The Spleen tissue lysate of Mouse(40ug)

Bioworld Molecular Weight: ~ 40 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: PAX5; BSAP

Immunogen: Synthetic peptide, corresponding to the N-terminus of Human Pax-5.

Conjugate: Unconjugated

Modification: Unmodified