Product Description

PAX8 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS91027-50, BS91027-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q06710(Human) Q00288(Mouse) P51974(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: ICC/IF, IHC

All Applications: ICC:1:50-1:200
IHC:1:50-1:200

Background: The Pax family encodes transcription factors that function during embryogenesis and regulate the temporal and position-dependent differentiation of cells. Pax-8 is expressed in the developing and adult thyroid, the developing secretory system and at lower levels, in the adult kidney. Pax-8 complexes with TTF-1 and TTF-2 to induce thyroid follicular cell differentiation and thyroid hormone biosynthesis by regulating the expression of sodium iodide symporter (NIS), thyroid peroxidase (TPO), thyroglobulin (TG) and the thyrotropin receptor (TSHR). Treatment of FRTL-5 cells with TGFβ1 decreases Pax-8 mRNA levels and Pax-8 DNA binding activity, which suppresses the expression of TG and the formation of thyrocytes. Patients who have autosomal dominant mutations of the Pax-8 gene develop thyroid dysgenesis. The Pax-8 gene produces six isoforms, A to F, that are generated by alternative splicing and differ in their carboxy-terminal regions. The Pax-8 isoforms display different DNA binding capacities and are thought to be functionally distinct. The gene which encodes Pax-8 maps to human chromosome 2q12-q14.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: PAX8 polyclonal antibody detects endogenous levels of PAX8 protein.

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: ICC staining PAX8 in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS., Immunohistochemical analysis of paraffin-embedded human thyroid tissue using anti-PAX8 antibody. Counter stained with hematoxylin.