Product Description

PBF (R151) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS2285-50, BS2285-100

Sizes: 50µl, 100µl

Swiss-Prot: P53801

Host: Rabbit

Reactivity: Human

Applications: WB, IHC

Background: PBF, also known as PTTG1IP (pituitary tumor-transforming 1 interacting protein), is a 180 amino acid single-pass type I membrane protein that localizes both the cytoplasm and the nucleus and contains a coiled-coil domain. Expressed ubiquitously, PBF interacts with PTTG and is thought to facilitate the nuclear translocation of PTTG, thereby allowing the PTTG-dependent transcriptional activation of fibroblast growth factor (FGF). The gene encoding PBF maps to human chromosome 21, which houses approximately 300 genes and comprises nearly 1.5% of the human genome. Chromosome 21-associated disorders include Alzheimer’s disease, amyotrophic lateral sclerosis and, most notably, Down syndrome (also known as trisomy 21).

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: PBF (R151) polyclonal antibody detects endogenous levels of PBF protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of PBF (R151) pAb at 1:500 dilution
Lane 1: Panc1 whole cell lysate (40ug)
Lane 2: SGC7901 whole cell lysate (40ug)
Lane 3: A375 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of PBF (R151) pAb in paraffin-embedded human colorectal carcinoma tissue at 1:50.

Bioworld Molecular Weight: ~ 22 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: PTTG binding factor; PTTG-binding factor; PTTG; Pttg1ip; PBF; PTTG1IP

Immunogen: Synthetic peptide, corresponding to amino acids 130-180 of Human PBF.

Conjugate: Unconjugated

Modification: Unmodified