Product Description

PC-PLD2 (phospho Tyr169) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS65372-50, BS65372-100

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Swiss-Prot: O14939

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC-P, IF (paraffin section), ELISA

All Applications: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.

Background: phospholipase D2(PLD2) Homo sapiens The protein encoded by this gene catalyzes the hydrolysis of phosphatidylcholine to phosphatidic acid and choline. The activity of the encoded enzyme is enhanced by phosphatidylinositol 4,5-bisphosphate and ADP-ribosylation factor-1. This protein localizes to the peripheral membrane and may be involved in cytoskeletal organization, cell cycle control, transcriptional regulation, and/or regulated secretion. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2011],

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Phospho-PC-PLD2 (Y169) Polyclonal Antibody detects endogenous levels of PC-PLD2 protein only when phosphorylated at Y169.

Bioworld Molecular Weight: ~95 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using PLD2 (Phospho-Tyr169) Antibody, Immunohistochemistry analysis of paraffin-embedded human brain, using PLD2 (Phospho-Tyr169) Antibody. The picture on the right is blocked with the phospho peptide., Western blot analysis of lysates from Jurkat cells treated with TNF 20ng/ml 30', using PLD2 (Phospho-Tyr169) Antibody. The Lane on the right is blocked with the phospho peptide.

Alternative Name: PLD2; Phospholipase D2; PLD 2; hPLD2; Choline phosphatase 2; PLD1C; Phosphatidylcholine-hydrolyzing phospholipase D2

Immunogen: The antiserum was produced against synthesized peptide derived from human PLD2 around the phosphorylation site of Tyr169. AA range:136-185

Conjugate: Unconjugated

Modification: Phosphorylation