PCB Polyclonal Antibody-BS5841

PCB Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS5841-50, BS5841-100

Sizes: 50µl, 100µl

Swiss-Prot: P11498

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

Background: Pyruvate carboxylase (PCB) is a biotinylated mitchondrial enzyme that catalyzes the synthesis of oxaloacetate from pyruvate in a tissue specific manner. In addition to covalently binding the biotin cofactor, PCB contains consensus sequences for the attachment of ATP and the substrate pyruvate. The PCB gene is located on the long arm of chromosome 11. Mutations in PCB metabolism (pyruvate carboxylase deficiency) are known to cause lactic acidosis, hypoglycemia and mental retardation.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: PCB polyclonal antibody detects endogenous levels of PCB protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of PCB polyclonal antibody at 1:500 dilution
Lane 1: HEK293T cell lysate
Lane 2: NIH-T3 cell lysate
Lane 3: H9C2 cell lyate, Immunohistochemistry (IHC) analyzes of PCB pAb in paraffin-embedded human liver carcinoma tissue at 1:50.showing cytoplasmic staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Bioworld Molecular Weight: ~ 130 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Pyruvate carboxylase, mitochondrial; Pyruvic carboxylase; PCB; PC

Immunogen: Synthetic peptide, corresponding to amino acids 400-445 of Human PCB.

Conjugate: Unconjugated

Modification: Unmodified