PCIF1 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS77676-50, BS77676-100
Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q9H4Z3
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, IHC, IP
All Applications: WB,1:500 - 1:2000 | IHC,1:50 - 1:200 | IP,1:50 - 1:200
Background: Cap-specific adenosine methyltransferase that catalyzes formation of N(6,2'-O-dimethyladenosine cap (m6A(m by methylating the adenosine at the second transcribed position of capped mRNAs. Recruited to the early elongation complex of RNA polymerase II (RNAPII via interaction with POLR2A and mediates formation of m6A(m co-transcriptionally.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: Unmodification
Extra Notes: Western blot analysis of HeLa, using PCIF1 antibody at 1:2470 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 20s.
Note: For research use only, not for use in diagnostic procedure.
Bioworld Molecular Weight: 80KDa
Alternative Name: CAPAM; hCAPAM; hPCIF1; C20orf67; PPP1R121; PCIF1
Immunogen: Recombinant fusion protein of human PCIF1(NP_071387.1).
Conjugate: Unconjugated
Modification: Unmodified