PCMT1 Polyclonal Antibody-BS8353

PCMT1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS8353-50, BS8353-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P22061

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IF/ICC, IP,

All Applications: WB,1:500 - 1:2000 | IF/ICC,1:50 - 1:100 | IP,1:50 - 1:200

Background: This gene encodes a member of the type II class of protein carboxyl methyltransferase enzymes. The encoded enzyme plays a role in protein repair by recognizing and converting D-aspartyl and L-isoaspartyl residues resulting from spontaneous deamidation back to the normal L-aspartyl form. The encoded protein may play a protective role in the pathogenesis of Alzheimer's disease, and single nucleotide polymorphisms in this gene have been associated with spina bifida and premature ovarian failure. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Polyclonal Antibodies

Bioworld Molecular Weight: 25kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using PCMT1 antibody.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s., Immunofluorescence analysis of HeLa cells using PCMT1 antibody. Blue: DAPI for nuclear staining., Immunoprecipitation analysis of 200ug extracts of HepG2 cells using 1ug PCMT1 antibody. Western blot was performed from the immunoprecipitate using PCMT1 antibody at a dilution of 1:1000.

Alternative Name: PCMT1; PIMT

Immunogen: Recombinant fusion protein of human PCMT1(NP_001238978.1).

Conjugate: Unconjugated

Modification: Unmodified