PCNA Mouse Monoclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: MB9012-50, MB9012-100
Product: Mouse IgG1, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P12004
Host: Mouse
Reactivity: Human, Mouse, Rat
Applications: WB, IHC FC
All Applications: WB: 1:1000-1:2000
IHC: 1:200
FC:1:100
Background: The proliferating cell nuclear antigen (PCNA), a protein synthesized in early G1 and S phases of the cell cycle, functions in cell cycle progression, DNA replication, and DNA repair. In early S phase, PCNA exhibits granular distribution and is absent from the nucleoli, however, in late S phase, it relocates to the nucleoli. PCNA exists in two basic forms, one involved in ongoing DNA replication. which localizes specifically to the nucleus, and a second, soluble form, not implicated in constant synthesis. Interestingly, the latter form degrades in the presence of organic solvents, rendering it undetectable by histological methods in tissues using organic fixatives, and thus also providing a method of visualizing only the synthesizing form.
Purification and Purity: Protein A affinity purified
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: This antibody detects endogenous levels of PCNA and does not cross-react with related proteins.
Bioworld Molecular Weight: ~ 29, 36 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot (WB) analysis of PCNA Mouse mAb at 1:1000 dilution
Lane 1: L929 whole cell lysate
Lane 2: MCF7 whole cell lysate
Lane 3: PC12 whole cell lysate
Lane 4: Raji whole cell lysate
Lane 5: F9 whole cell lysate
Lane 6: A549 whole cell lysate, Immunohistochemical analysis of paraffin-embedded human stomach carcinoma tissue using anti-PCNA antibody. Counter stained with hematoxylin.
Alternative Name: Proliferating cell nuclear antigen; Cyclin
Immunogen: This antibody is produced by immunizing mice with recombinant protein corresponding to a region of PCNA.
Conjugate: Unconjugated
Modification: Unmodified