PCNA Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS91036-50, BS91036-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P12004(Human) P17918(Mouse) P04961(Rat)
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, ICC/IF, IHC, IP, , FC
All Applications: WB:1:1,000-1:5,000
ICC:1:50-1:200
IHC:1:100-1:500
FC:1:50-1:100
Background: The proliferating cell nuclear antigen (PCNA), a protein synthesized in early G1 and S phases of the cell cycle, functions in cell cycle progression, DNA replication and DNA repair. In early S phase, PCNA exhibits granular distribution and is absent from the nucleoli; however, in late S phase, it relocates to the nucleoli. PCNA exists in two basic forms: one involved in ongoing DNA replication, which localizes specifically to the nucleus, and a second, soluble form, not implicated in constant synthesis. Interestingly, the latter form degrades in the presence of organic solvents, rendering it undetectable by histological methods in tissues using organic fixatives, and thus also providing a method of visualizing only the synthesizing form.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: PCNA polyclonal antibody detects endogenous levels of PCNA protein.
Bioworld Molecular Weight: 29 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of PCNA on different lysates using anti-PCNA antibody at 1/1,000 dilution.
Positive control:
Lane 1: Hela
Lane 2: 293
Lane 3: A431, Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PCNA antibody. Counter stained with hematoxylin.
Alternative Name: PCNA, ATLD2, cb16, Cyclin, DNA polymerase delta auxiliary protein, etID36690.10, fa28e03, fb36g03, HGCN8729, MGC8367, Mutagen-sensitive 209 protein, OTTHUMP00000030189, OTTHUMP00000030190, PCNA, Pcna/cyclin, PCNA, PCNAR, Polymerase delta accessory protein, Proliferating cell nuclear antigen, wu:fa28e03, wu:fb36g03,
Immunogen: recombinant protein
Conjugate: Unconjugated
Modification: Unmodified