PCNA Polyclonal Antibody
Sizes: 60μL, 120μL, 200μL
Catalogue Numbers: E-AB-70004-60, E-AB-70004-120, E-AB-70004-200
Citations, Manuals and MSDS Available upon request.
Abbreviation: Proliferating Cell Nuclear Antigen
Target Synonym: ATLD2; cb16; Cyclin; DNA polymerase delta auxiliary protein; etID36690.10; fa28e03; fb36g03; HGCN8729; MGC8367; Mutagen-sensitive 209 protein; OTTHUMP00000030189; OTTHUMP00000030190; PCNA; Pcna/cyclin; PCNA; PCNAR; Polymerase delta accessory protein; Proliferating cell nuclear antigen; wu:fa28e03; wu:fb36g03
Research Areas: Cancer, Cell Biology, Epigenetics and Nuclear Signaling, Tags and Cell Markers
Conjugation: Unconjugated
Host: Rabbit
Species Reactivity: Human, Mouse, Rat
Application: WB, IHC
Isotype: IgG
Clonality: Polyclonal
UNIProt ID: P12004,P17918,P04961
Background: Proliferating Cell Nuclear Antigen, commonly known as PCNA, is a protein that acts as a processivity factor for DNA polymerase δ in eukaryotic cells. This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. PCNA induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. It has to be loaded onto DNA in order to be able to stimulate APEX2. PCNA protein is highly conserved during evolution; the deduced amino acid sequences of rat and human differ by only 4 of 261 amino acids. PCNA has been used as loading control for proliferating cells.
Concentration: 270 μg/mL
Immunogen: KLH conjugated Synthetic peptide corresponding to Mouse PCNA
Buffer: PBS with 0.02% sodium azide, 1% protective protein and 50% glycerol, pH7.4
Purification Method: Affinity purification
Dilution: WB 1:1000-1:3000, IHC 1:300-1:800
Calculated MW: 33kDa
Observed MW: 33kDa
Storage: -20°C/One year. Avoid freeze / thaw cycles.
Shipping: Ice bag
Research Use Only