PCOLCE Polyclonal Antibody-BS76233

PCOLCE Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS76233-50, BS76233-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q15113

Host: Rabbit

Reactivity: Human

Applications: WB

All Applications: WB,1:200 - 1:2000

Background: Fibrillar collagen types I-III are synthesized as precursor molecules known as procollagens. These precursors contain amino- and carboxyl-terminal peptide extensions known as N- and C-propeptides, respectively, which are cleaved, upon secretion of procollagen from the cell, to yield the mature triple helical, highly structured fibrils. This gene encodes a glycoprotein which binds and drives the enzymatic cleavage of type I procollagen and heightens C-proteinase activity.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Unmodification

Extra Notes: Western blot analysis of extracts of U-251MG cells, using PCOLCE antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.

Note: For research use only, not for use in diagnostic procedure.

Bioworld Molecular Weight: 48kDa

Alternative Name: PCOLCE; PCPE; PCPE-1; PCPE1

Immunogen: Recombinant fusion protein of human PCOLCE(NP_002584.2).

Conjugate: Unconjugated

Modification: Unmodified