Product Description

PCP4 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS65683-50, BS65683-100

Product: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Swiss-Prot: P48539

Host: Rabbit

Reactivity: Human, Mouse, Rat,Cow,Sheep,

Applications: IHC-P

All Applications: IHC-P=1:100-500

Background: PCP-4 is a calmodulin (CaM) regulatory protein that is highly expressed in neuronal cells. Through its IQ motif, PCP-4 mediates both the calcium-dependent binding properties of CaM and the rates of association and dissociation of calcium from the C-terminal domain of CaM. The IQ motif contains a serine residue which can be phosphorylated by all isoforms of protein kinase C (PKC). PCP-4 is implicated in uterine leiomyomas, as well as in neurodegenerative disorders such as Alzheimer’s disease and Huntington's disease. Additionally, overexpression of PCP-4 is thought to play a role in cerebellar hypoplasia, a key feature of Down syndrome.

Purification and Purity: affinity purified by Protein A

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: PCP4 Polyclonal Antibody detects endogenous levels of PCP4 protein.

Bioworld Molecular Weight: 7kD

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes;Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PCP4) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C

Alternative Name: Brain specific antigen PCP 4; Brain specific polypeptide PEP 19; Brain specific polypeptide PEP19; PEP19; Purkinje cell protein 4; PCP-4; PCP4_HUMAN; PEP-19.

Immunogen: KLH conjugated synthetic peptide derived from human PCP4

Conjugate: Unconjugated

Modification: Unmodified