Product Description

PDE4C Polyclonal Antibody

Catalogue Numbers: BS5844-50, BS5844-100

Sizes: 50µl, 100µl

Alternative Name: cAMP-specific 3',5'-cyclic phosphodiesterase 4C; DPDE1; PDE21; DPDE1

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q08493

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB ICC

All Applications: WB: 1:500~1:1000
ICC: 1:50~1:200

Background: Phosphodiesterases (PDE) hydrolyze cAMP to 5’AMP and thus play a critical role in the regulation of intracellular cAMP. Division of the PDE superfamily by sequence homology and enzymatic properties yields 11 PDE families. A unique upstream conserved region (UCR) characterizes the PDE4 family. Four separate genes (A-D) encode the PDE4 enzymes, and alternative splicing generates short or long isoforms of each gene. Long PDE4 isoforms contain both UCR1 and UCR2 while short PDE4 isoforms possess only UCR2. Both UCR domains are necessary for dimerization of PDE4 isoforms. The human PDE4C gene maps to chromosome 19p13.1. The splice variants include isoforms PDE4C1-7.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: PDE4C polyclonal antibody detects endogenous levels of PDE4C protein.

Bioworld Molecular Weight: ~ 80 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of PDE4C polyclonal antibody at 1:500 dilution
Lane 1: HEK293T cell lysate
Lane 2: Mouse liver tissue lysate
Lane 3: H9C2 cell lysate

Immunogen: Synthetic peptide, corresponding to amino acids 333-378 of Human PDE4C.

Conjugate: Unconjugated

Modification: Unmodified