Product Description

PDE4D (H47) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS1572-50, BS1572-100

Sizes: 50µl, 100µl

Swiss-Prot: Q08499

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

Background: Phosphodiesterases (PDE) hydrolyze cAMP to 5’AMP and thus play a critical role in the regulation of intracellular cAMP. Division of the PDE superfamily by sequence homology and enzymatic properties yields 11 PDE families. A unique upstream conserved region (UCR) characterizes the PDE4 family. Four separate genes (A-D) encode the PDE4 enzymes, and alternative splicing generates short or long isoforms of each gene. Long PDE4 isoforms contain both UCR1 and UCR2 while short PDE4 isoforms possess only UCR2. Both
UCR domains are necessary for dimerization of PDE4 isoforms. The human PDE4D gene maps to chromosome 5q12.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: PDE4D (H47) polyclonal antibody detects endogenous levels of PDE4D protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of PDE4D (H47) polyclonal antibody at 1:500 dilution
Lane 1: HEK293T whole cell lysate
Lane 2: sp2/0 whole cell lysate
Lane 3: H9C2 whole cell lysate, Immunohistochemistry (IHC) analyzes of PDE4B/C/D (H47) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Bioworld Molecular Weight: ~ 91 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: cAMP-specific 3',5'-cyclic phosphodiesterase 4D; DPDE3; PDE43; PDE4D; DPDE3

Immunogen: Synthetic peptide, corresponding to amino acids 30-70 Human PDE4D.

Conjugate: Unconjugated

Modification: Unmodified