PDF Rabbit pAb
Sizes: 20μL, 100μL
Catalogue Numbers: A10458-20, A10458-100
Citations, Manuals and MSDS Available upon request.
Background: Protein synthesis proceeds after formylation of methionine by methionyl-tRNA formyl transferase (FMT) and transfer of the charged initiator f-met tRNA to the ribosome. In eubacteria and eukaryotic organelles the product of this gene, peptide deformylase (PDF), removes the formyl group from the initiating methionine of nascent peptides. In eubacteria, deformylation of nascent peptides is required for subsequent cleavage of initiating methionines by methionine aminopeptidase. The discovery that a natural inhibitor of PDF, actinonin, acts as an antimicrobial agent in some bacteria has spurred intensive research into the design of bacterial-specific PDF inhibitors. In human cells, only mitochondrial proteins have N-formylation of initiating methionines. Protein inhibitors of PDF or siRNAs of PDF block the growth of cancer cell lines but have no effect on normal cell growth. In humans, PDF function may therefore be restricted to rapidly growing cells.
Category: Polyclonal Antibodies
Applications: WB, ELISA
Cross-reactivity: Human, Mouse, Rat
Protein Weight: 27kDa
Observed Molecular Weight: 20kDa
Immunogen: Recombinant protein (or fragment)
Species: Human
GeneID: 64146
SWISS: Q9HBH1
Alternate Names: PDF
Source: Rabbit
Isotype: IgG
Purity: Affinity purification
Storage: Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.
Recommended Dilutions: WB, 1:500 - 1:2000 ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Research Areas: Epigenetics Nuclear Signaling, Cancer, Signal Transduction, Cell Biology Developmental Biology, Cell Cycle, Endocrine Metabolism, Mitochondrial metabolism, Mitochondrial markers, Amino acid metabolism, Nucleotide metabolism.
NCBI Alias: PDF
Research Use Only