PDGF-B (L48) Polyclonal Antibody-BS1290

PDGF-B (L48) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS1290-50, BS1290-100

Sizes: 50µl, 100µl

Swiss-Prot: P01127

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

Background: PDGF is a mitogen for mesenchyme- and glia-derived cells. It consists of two disulfide-bonded polypeptide chains, A and B, and occurs as three isoforms, PDGF AA, PDGF AB and PDGF BB. The three isoforms bind with different affinities to two receptor types, α and β, which are structurally related and endowed with protein-tyrosine kinase domains. Ligand binding induces activation of the receptor kinases by formation of receptor dimers; the A subunit of PDGF binds only to α receptors with high affinity, whereas the B subunit can bind to both α and β receptors. Evidence suggests that PDGF may function as a neurotrophic factor.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: PDGFB (L48) polyclonal antibody detects endogenous levels of PDGFB protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of PDGF-B (L48) pAb at 1:500 dilution
Lane 1: HepG2 whole cell lysate (40ug)
Lane 2: 3T3-L1 whole cell lysate (40ug)
Lane 3: H9C2 whole cell lysate (40ug)
Lane 4: H1792 whole cell lysate (40ug)
Lane 5: Hela whole cell lysate (40ug)
Lane 6: HEK293T whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of PDGF-B (L48) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.

Bioworld Molecular Weight: ~ 27 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Platelet-derived growth factor subunit B; PDGF subunit B; PDGF-2; Platelet-derived growth factor B chain; Platelet-derived growth factor beta polypeptide; Proto-oncogene c-Sis; Becaplermin; PDGFB; PDGF2; SIS

Immunogen: Synthetic peptide, corresponding to amino acids 21-70 of Human PDGF-B.

Conjugate: Unconjugated

Modification: Unmodified