PDHA1 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS91040-50, BS91040-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P08559(Human) P35486(Mouse) P26284(Rat)
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, ICC/IF, IHC, IP, , FC
All Applications: WB:1:1,000-1:5,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100
Background: The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial matrix enzyme complex that functions as the primary link between glycolysis and the tricarboxylic acid (TCA) cycle by catalyzing the irreversible conversion of pyruvate into acetyl-CoA. The E1 enzyme of the PDH complex is made up of a heterotetramer of two α and two β subunits. The E1-α subunit (PDH-E1α) contains the E1 active site and plays a key role in the function of the PDH complex. The PDH complex is regulated by phosphorylation and dephosphorylation of PDH-E1α. The gene encoding for PDH-E1α maps to chromosome Xp22.12, and a 20bp deletion in the last exon of this gene is sufficient to cause PDH deficiency, which causes a broad range of symptoms including the development of seizures, mental retardation and spasticity, as well as intermittent episodes of lactic acidosis associated with cerebellar ataxia.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: PDHA1 polyclonal antibody detects endogenous levels of PDHA1 protein.
Bioworld Molecular Weight: 43 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of PDHA1 on different lysates using anti-PDHA1 antibody at 1/1,000 dilution.
Positive control:
Lane 1: 293T
Lane 2: A431
Lane 3: Mouse heart, ICC staining PDHA1 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: PDHA1, ODPA, PDH, PDHA, PDHA1, PDHCE1A, PDHE1 A type I, PDHE1-A type I, PHE1A, Pyruvate Dehydrogenase (lipoamide) alpha 1, Pyruvate dehydrogenase complex, E1 alpha polypeptide 1, Pyruvate Dehydrogenase E1 alpha, Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial,
Immunogen: recombinant protein
Conjugate: Unconjugated
Modification: Unmodified