PDHA2 Polyclonal Antibody-BS76068

PDHA2 Polyclonal Antibody

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Sizes: 50µl, 100µl

Catalogue Numbers: BS76068-50, BS76068-100

Swiss-Prot: P35487

Host: Rabbit

PDHA2 Polyclonal Antibody-BS76068

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Reactivity: Human, Mouse, Rat

Applications: WB, IF/ICC

All Applications: WB,1:500 - 1:2000 | IF/ICC,1:50 - 1:100

Background: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2, and thereby links the glycolytic pathway to the tricarboxylic cycle.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Unmodification

Bioworld Molecular Weight: 43kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using Pdha2 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s., Immunofluorescence analysis of L929 cells using PDHA2 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Alternative Name: PDHA2; PDHAL; Pdha2

Immunogen: Recombinant fusion protein of mouse PDHA2(NP_032837.1).

Modification: Unmodified

Conjugate: Unconjugated