PDI Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS91042-50, BS91042-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q13087
Host: Rabbit
Reactivity: Human, Mouse, rat
Applications: WB, ICC IHC
All Applications: WB:1:500-1:1,000
ICC:1:200
IHC:1:200
Background: Protein disulfide isomerase or PDI is an enzyme in the endoplasmic reticulum in eukaryotes that catalyzes the formation and breakage of disulfide bonds between cysteine residues within proteins as they fold. Another major function of PDI relates to its activity as a chaperone; i.e., it aids wrongly folded proteins to reach a correctly folded state without the aid of enzymatic disulfide shuffling. PDI has been found to be involved in the breaking of bonds on the HIV gp120 protein during HIV infection of CD4 positive cells, and is required for HIV infection of lymphocytes and monocytes.
Purification and Purity: Peptide affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: PDI polyclonal antibody detects endogenous levels of PDI protein.
Bioworld Molecular Weight: 58 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of PDI on different cell lysates using anti- PDI antibody at 1/1000 dilution.
Positive control:
Lane 1: Hela
Lane 2: MCF-7
Lane 3: PANC
Lane 4: HepG2
Lane 5: HUVEC
Lane 6: NIH/3T3
Lane 7: L929
Lane 8: F9
Lane 9: Jurkat
Lane 10: A431
Lane 11: A549
Lane 12: Mouse liver
Lane 13: Mouse kidney
Lane 14: Mouse brain
Lane 15: Human kidney
Lane 16: Human brain
Lane 17: Human liver, ICC staining PDI in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: PDIp, Pancreas specific protein disulfide isomerase, Pancreas-specific protein disulfide isomerase, Pancreatic protein disulfide isomerase, PDA2, PDI, PDIA2, PDIA2, PDIp, PDIR, Protein disulfide isomerase A2, Protein disulfide isomerase, Protein disulfide isomerase associated 2, Protein disulfide isomerase family A member 2, Protein disulfide isomerase pancreatic, Protein disulfide-isomerase A2, Rho GDP dissociation inhibitor gamma,
Immunogen: peptide
Conjugate: Unconjugated
Modification: Unmodified