Product Description

PDIA6 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS91044-50, BS91044-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q15084(Human) Q922R8(Mouse) Q63081(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC/IF FC

All Applications: WB:1:1,000-1:2,000
FC:1:50-1:100
ICC:1:100-1:500

Background: Endoplasmic reticulum proteins (ERps) are widely expressed proteins that localize to the ER and may act as proteases, protein disulfide isomerases, thiol-disulfide oxidases or phospholipases. ERp5, also known as PDIA6 (protein disulfide isomerase family A, member 6) or TXNDC7 is a 440 amino acid protein that contains two thioredoxin domains and belongs to the protein disulfide isomerase family. Localized to the melanosome, as well as to the lumen of the endoplasmic reticulum, ERp5 functions to catalyze the rearrangement of disulfide bonds in a variety of different proteins. Via its catalytic activity, ERp5 is able to reduce the disulfide bond that binds MICA to tumor cells, thereby releasing MICA and reducing the rate of tumor expansion. Multiple isoforms of ERp5 exist due to alternative splicing events.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: PDIA6 polyclonal antibody detects endogenous levels of PDIA6 protein.

Bioworld Molecular Weight: 48 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of PDIA6 on different lysates using anti-PDIA6 antibody at 1/1,000 dilution.
Positive control:
Lane 1: HepG2
Lane 2: K562, ICC staining PDIA6 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: ER protein 5, ERp5, PDIA6, Endoplasmic reticulum protein 5, ER protein 5, ERp5, P5, Pdia6, PDIA6, Protein disulfide isomerase A6, Protein disulfide isomerase associated 6, Protein disulfide isomerase family A member 6, Protein disulfide isomerase P5, Protein disulfide isomerase related protein, Protein disulfide-isomerase A6, Thioredoxin domain containing 7 (protein disulfide isomerase), Thioredoxin domain containing protein 7, Thioredoxin domain-containing protein 7, TXNDC7,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified