Product Description

PDK1 (phospho Ser241) Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: RA28639-50, RA28639-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: catalytic activity:ATP + a protein = ADP + a phosphoprotein., function:Phosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development (By similarity). Isoform 3 is catalytically inactive., PTM:Phosphorylated on tyrosine and serine/threonine. Phosphorylation on Ser-241 in the activation loop is required for full activity. PDK1 itself can autophosphorylate Ser-241, leading to its own activation., similarity:Belongs to the protein kinase superfamily., similarity:Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PDK1 subfamily., similarity:Contains 1 PH domain., similarity:Contains 1 protein kinase domain., subcellular location:Membrane-associated after cell stimulation leading to its translocation. Tyrosine phosphorylation seems to occur only at the plasma membrane., subunit:Interacts with TUSC4., tissue specificity:Appears to be expressed ubiquitously.,

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC-p, IF (paraffin section), ELISA

Alternate Names: PDPK1, PDK1, 3-phosphoinositide-dependent protein kinase 1, hPDK1

Clonality: Polyclonal

Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.

Molecular Weight: 63

Protein Molecular Weight (kDa): 63

Storage: -20°C/1 year

Human Gene ID: 5170

Human SwissProt Number: O15530

Condition: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Research Use Only