Product Description

PDK1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS91045-50, BS91045-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q15118(Human) Q8BFP9(Mouse) Q63065(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IP, , FC

All Applications: WB:1:500-1:2,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100

Background: Mitochondrial pyruvate dehydrogenase (PDH) catalyzes the oxidative decarboxylation of pyruvate and plays a central role in the regulation of homeostasis of carbohydrate fuels in mammals. PDH activity is controlled by a phosphorylation/dephosphorylation cycle, phosphorylation leading to inactivation and dephosphorylation leading to reactivation of PDH. The phosphorylation of PDH is catalyzed by pyruvate dehydrogenase kinase (PDK), the activity of which is stimulated by the products of PDH catalysis. PDK1 consists of alpha and beta subunits; the kinase activity resides in the alpha subunit. Three PDK isoenzymes have been identified in humans (PDK1, 2 and 3) and two have been identified in rodent (PDK1 and 2).

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: PDK1 polyclonal antibody detects endogenous levels of PDK1 protein.

Bioworld Molecular Weight: 49 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of PDK1 on rat heart tissue lysate using anti-PDK1 antibody at 1/1,000 dilution., ICC staining PDK1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: PDH kinase 1, PDK1, PDHK1,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified