PDK1 Polyclonal Antibody - RA28641

PDK1 Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: RA28641-50, RA28641-100

Citations, Manuals and MSDS Available upon request.

Background: catalytic activity:ATP + a protein = ADP + a phosphoprotein.,function:Phosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development (By similarity) . Isoform 3 is catalytically inactive.,PTM:Phosphorylated on tyrosine and serine/threonine. Phosphorylation on Ser-241 in the activation loop is required for full activity. PDK1 itself can autophosphorylate Ser-241, leading to its own activation.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PDK1 subfamily.,similarity:Contains 1 PH domain.,similarity:Contains 1 protein kinase domain.,subcellular location:Membrane-associated after cell stimulation leading to its translocation. Tyrosine phosphorylation seems to occur only at the plasma membrane.,subunit:Interacts with TUSC4.,tissue specificity:Appears to be expressed ubiquitously.,

Condition: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage Instructions: -20°C/1 year

Recommended dilutions: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.

Alternative Names: PDPK1; PDK1; 3-phosphoinositide-dependent protein kinase 1; hPDK1

Applications: WB; IHC-p; IF (paraffin section) ; ELISA

Species Cross-Reactivity: Human; Mouse; Rat; Monkey

GeneID (Human): 5170

Protein MW (KDa): 60

SWISS: O15530

Source: Rabbit

Research Use Only