PDK2 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS91046-50, BS91046-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q15119(Human)
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, IHC FC IP,
All Applications: WB:1:500
IHC:1:50-1:200
FC:1:50-1:100
Background: Pyruvate dehydrogenase kinase family members (PDK1, 2, 3, 4) are serine kinases that catalyze the phosphorylation of the E1α subunit of the pyruvate dehydrogenase complex (PDC). PDC activity is controlled through phosphorylation and dephosphorylation of the E1α subunit, which leads to inactivation and reactivation, respectively. The core of PDC is composed of sixty dihydrolypoyl acetyltransferase (E2) subunits that bind directly to PDK2 and enhance PDK2 kinase activity. Upregulation of PDK isoenzymes occurs during starvation conditions, rerouting acetyl-CoA generation by facilitating fatty acid oxidation. PDKs contain five conserved regions and are mechanistically similar to bacterial His-kinases, in that both require Histidine residues for activity. In mammals, transcripts for PDK2 are ubiquitously expressed with high levels in heart and skeletal muscle and decreased levels in spleen and lung.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: PDK2 polyclonal antibody detects endogenous levels of PDK2 protein.
Bioworld Molecular Weight: 46 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of PDK2 on rat heart tissue lysate using anti-PDK2 antibody at 1/500 dilution., Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-PDK2 antibody. Counter stained with hematoxylin.
Alternative Name: PDH kinase 2, PDKII, PDK2, PDHK2, [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 2, mitochondrial, PDHK2, PDK2, PDK2, Pyruvate dehydrogenase kinase isoform 2, Pyruvate dehydrogenase kinase, isozyme 2, Pyruvate dehydrogenase lipoamide kinase isozyme 2, mitochondrial,
Immunogen: Recombinant protein
Conjugate: Unconjugated
Modification: Unmodified