PDP1 Polyclonal Antibody
Catalogue Numbers: BS60681-50, BS60681-100
Sizes: 50µl, 100µl
Alternative Name: [Pyruvate dehydrogenase [acetyl-transferring]]-phosphatase 1, mitochondrial; PDP 1; Protein phosphatase 2C; Pyruvate dehydrogenase phosphatase catalytic subunit 1; PDPC 1; PDP1; PDP; PPM2C
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q9P0J1
Host: Rabbit
Reactivity: Human, Mouse
Applications: WB
All Applications: WB: 1:500~1:1000
Background: Pyruvate dehydrogenase phosphatase (PDP) is a serine phosphatase that catalyzes the dephosphorylation and reactivation of the alpha subunit of the E1 component of the mitochondrial pyruvate dehydrogenase multienzyme complex. PDP is a heterodimer that consists of catalytic and regulatory subunits. PDPc (pyruvate dehydrogenase phosphatase, catalytic subunit 1), also known as Protein phosphatase 2C, is a 537 amino acid protein that is localized within the mitochondrial matrix. PDPc is stimulated by calcium binding and utilizes two magnesium ions as cofactors. PDPc efficiently dephosphorylates all three phosphorylation sites located on the alpha chain of the E1 component, which simultaneously activates pyruvate dehydrogenase to convert pyruvate to acetyl-CoA for utilization in the Kreb’s Cycle. Defects in the gene encoding PDPc are the cause of pyruvate dehydrogenase phosphatase deficiency, which results in lactic acidosis and neurological dysfunction.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: PDP1 polyclonal antibody detects endogenous levels of PDP1 protein.
Bioworld Molecular Weight: ~ 61 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot (WB) analysis of PDP1 polyclonal antibody at 1:500 dilution
Lane 1: A549 whole cell lysate
Lane 2: sp2/0 whole cell lysate
Lane 3: MCF-7 whole cell lysate
Immunogen: A synthetic peptide corresponding to residues in Human PDP1
Conjugate: Unconjugated
Modification: Unmodified