Product Description

PDPK1 (phospho-S241) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS4155-50, BS4155-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: PDK1 (3 Phosphoinositide Dependent Protein Kinase 1) phosphorylates AGC kinases. PDK1 activates conventional PKC and PKC zeta through phosphorylation of critical threonine residues in the activation loop. PDK1 also phosphorylates Protein Kinase B (PKB) at threonine 308 in the presence of hosphatidylinositol-3, 4, 5-trisphosphate. Active Akt inactivates Glycogen Synthase Kinase 3 (GSK3), eventually leading to the dephosphorylation and activation of glycogen synthase, and the stimμlation of glycogen synthesis. Because of the role that PDK plays in insμlin-induced glycogen synthesis and PKC activation, it is a potentially important target for metabolic drug research.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC

Alternate Names: 3-phosphoinositide-dependent protein kinase 1, hPDK1, PDPK1, PDK1

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Phosphorylation

Immunogen: Synthetic phosphopeptide derived from human PDPK1 around the phosphorylation site of Serine 241.

Dilution: WB: 1:500~1:1000
IHC: 1:50~1:200

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: p-PDPK1 (S241) polyclonal antibody detects endogenous levels of PDPK1 protein when phosphorylated at Ser241.

Molecular Weight: ~ 63 kDa

Pathway: Phosphoinosotode Signaling, Protein Kinase C Signaling, Translational Contral elF4 and p70 S6 Kinase, Inhibition of Apoptosis, Insμlin &Glucose Signaling,

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: O15530

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only