PE-Cy5.5 Anti-Mouse CD22 Antibody (Cy34.1)
Sizes: 25μg, 100μg
Catalogue Numbers: PC55-30016U-25, PC55-30016U-100
Citations, Manuals and MSDS Available upon request.
Target: Mouse CD22
Form: Liquid
Conjugation: PE-Cy5.5
Conjugation Information: PE-Cy5.5 is designed to be excited by the Blue (488 nm), Green (532 nm) and yellow-green (561 nm) lasers and detected using an optical filter centered near 690 nm (e.g., a 690/50 nm bandpass filter).
Host: Mouse
Clonality: Monoclonal
Clone ID: Cy34.1
Isotype: IgG1, κ
Storage: PBS with 0. 1% sodium azide, 1%BSA, pH 7.3, 2-8℃ for 12 months (Avoid repeated freeze / thaw cycles.)
Background: The Cy34.1 Monoclonal Antibody specifically binds to the B-lymphocyte differentiation antigen CD22 on strains having the Lyb-8.2 alloantigen (e.g., A, BALB/c, CBA, C3H/He, C57BL, C57L, C58, SJL, SWR, but not AKR, DBA/1, DBA/2, NZB, PL). CD22 is expressed at high levels on mature peripheral B lymphocytes (follicular and marginal zone), B-1 cells (CD5+ B cells), and plasma cells. It is a member of the Ig gene superfamily and associates with the B-cell antigen receptor. Its sialic acid- binding immunoglobulin-like lectin (siglec) extracellular region mediates B-cell adhesion to ligands on endothelial cells in the bone marrow. Its intracellular domain is phosphorylated after cross-linking of antigen receptor or MHC class II antigen. It is involved in negative regulation of B-cell activation and protection from autoimmunity. B-cell proliferative responses to LPS or anti-mouse Ig µ chain are augmented in the presence of Cy34.1 mAb.
Synonyms: Siglec2| B-cell receptor CD22| B-lymphocyte cell adhesion molecule| BL-CAM| CD22| Cd22| Lyb-8| Sialic acid-binding Ig-like lectin 2| Siglec-2| T-cell surface antigen Leu-14
Uniprot ID: P35329
Reactivity: Mouse
Tested Application: FC
Recommended Dilution: Each lot of this antibody is quality control tested by Flow cytometryometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1μg/10⁶ cells in 100 μL volume].
Gene ID: 12483
Category: Flow cytometryometry Antibodies
Research Use Only