PEA15 Polyclonal Antibody
Catalogue Numbers: BS61435-50, BS61435-100
Sizes: 50µl, 100µl
Alternative Name: Astrocytic phosphoprotein PEA-15; 15 kDa phosphoprotein enriched in astrocytes; Phosphoprotein enriched in diabetes; PED; PEA15
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q15121
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB
All Applications: WB: 1:500~1:1000
Background: PEA-15 (Phosphoprotein Enriched in Astrocytes) exists in an non-phosphorylated form (N), and two phosphorylated forms, Pa and Pb. PEA-15 is an endogenous substrate for PKC, which mediates the transition from Pa to Pb. The level of PEA-15 phosphorylation changes upon depolymerization or stabilization of tubulins, indicating that PEA-15 colocalizes with microtubules. The first 80 amino acids of PEA-15 correspond to the death effector domain (DED), which is a domain found in proteins that regulate apoptotic signaling pathways. The DED domain is necessary for PEA-15 to block Ras suppression. Although PEA-15 is predominantly expressed in the central nervous system, low levels of PEA-15 are expressed in liver and kidney, and higher levels in muscle. PEA-15 is also referred to as PED, Phosphoprotein Enriched in Diabetes, for its elevated expression in type 2 diabetic patients.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: PEA15 polyclonal antibody detects endogenous levels of PEA15 protein.
Bioworld Molecular Weight: ~ 15 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot (WB) analysis of PEA15 polyclonal antibody at 1:500 dilution
Lane 1: Hela whole cell lysate(30ug)
Lane 2: H9C2 whole cell lysate(30ug)
Lane 3: RAW264.7 whole cell lysate(30ug)
Lane 4: HEK293T whole cell lysate(30ug)
Immunogen: Synthetic peptide, corresponding to amino acids 85-130 of Human PEA15.
Conjugate: Unconjugated
Modification: Unmodified