PEBP2β (R33) Polyclonal Antibody-BS2307

PEBP2β (R33) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS2307-50, BS2307-100

Sizes: 50µl, 100µl

Swiss-Prot: Q13951

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF

Background: The transcription factor Polyomavirus enhancer binding protein 2 (PEBP2), also designated Osf2 (Osteoblast-specific transcription factor), CBFA1 (Core Binding Factor) and AML3 (Acute myeloid leukemia), is composed of two subunits, α and β, which are essential for the regulation of hematopoiesis and osteogenesis. The PEBP2α subunits, PEBP2αA, PEBP2αB and PEBP2αC, are encoded by three RUNX genes, all of which contain a 128-amino acid region homologous to the highly conserved Drosophila segmentation gene, runt. This region is involved in DNA binding and heterodimerization with the regulatory β subunit, which facilitates DNA binding of the α subunit. Both subunits are required for in vivo function; the disruption of either gene results in a lack of definitive hematopoiesis followed by embryo death in utero due to hemorrhage in the central nervous system.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: PEBP2β (R33) polyclonal antibody detects endogenous levels of PEBP2β protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of PEBP2β (R33) polyclonal antibody at 1:500 dilution
Lane 1: L02 whole cell lysate (40ug)
Lane 2: HEK293T whole cell lysate (40ug)
Lane 3: A549 whole cell lysate (40ug)
Lane 4: SGC-7901 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of PEBP2β (R33) pAb in paraffin-embedded human colorectal carcinoma tissue at 1:50.

Bioworld Molecular Weight: ~ 22 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Core-binding factor subunit beta; CBF-beta; CBF-β; CBFβ; Polyomavirus enhancer-binding protein 2 beta subunit; PEA2-β; PEA2β; PEA2-beta; PEBP2-β; PEBP2-beta; SL3-3 enhancer factor 1 subunit beta; SL3/AKV core-binding factor beta subunit; CBFB

Immunogen: Synthetic peptide, corresponding to amino acids 2-56 of Human PEBP2β.

Conjugate: Unconjugated

Modification: Unmodified