Product Description

PEN2 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS9960M-50, BS9960M-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9NZ42

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC ICC FC

All Applications: WB: 1:500-1:2000 IHC: 1:50-1:200
ICC: 1:50-1:200 FC: 1:50-1:100

Background: Four proteins comprise the gamma-secretase complex: presenilin, nicastrin, aph-1, and PEN-2. Together, these proteins mediate cell surface signaling pathways for a variety of type I membrane proteins, notably amyloid-beta precursor protein, a protein implicated in the development of Alzheimer's disease, via intramembrane proteolysis. The proteins assemble into a proteolytically active complex in the golgi/trans-golgi network (TGN) compartments. Assembly leads to autocleavage of presenilin into two subunits to create the active site of gamma-secretase, an important step in understanding the mechanisms involved in the etiology and possible treatment of Alzheimer's disease.

Purification and Purity: Protein A affinity purified.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: This antibody detects endogenous levels of PEN2 and does not cross-react with related proteins.

Bioworld Molecular Weight: ~ 12 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of PEN2 on mouse spleen tissue lysate using anti-PEN2 antibody at 1:2000 dilution., Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PEN2 antibody. Counter stained with hematoxylin., Flow cytometric analysis of A549 cells with PEN2 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Alternative Name: Gamma-secretase subunit PEN-2; Presenilin enhancer protein 2; PSENEN; PEN2; MDS033

Immunogen: Synthetic peptide corresponding to C-terminal human PEN2.

Conjugate: Unconjugated

Modification: Unmodified