Product Description

PER2/Period circadian Protein 2 Polyclonal Antibody

Product: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Sizes: 50µl, 100µl

Catalogue Numbers: BS65878-50, BS65878-100

Swiss-Prot: O15055

Host: Rabbit

PER2/Period circadian Protein 2 Polyclonal Antibody-BS65878

Storage and Stability: Store at 4°C short term. Aliquot and store at -51°C long term. Avoid freeze-thaw cycles.

Reactivity: Human, Mouse, Rat, Dog, Cow, Horse,

Applications: IHC-P

All Applications: IHC-P=1:100-500

Background: PER2, a mammalian homologue of the Drosophila period gene, shares a 40% homology with PER1 including the protein dimerization PAS domain. PER2 is a circadian regulator that may act as a transcription factor. It behaves as a negative element in circadian transcriptional loop. PER2 does not appear to bind DNA, suggesting indirect transcriptional inhibition. Expression oscillates with a 24 hour rhythm in the suprachiasmatic nucleus (SCN) and the whole eyes. Oscillations are maintained under constant darkness and are responsive to changes of the light/dark cycles. There is a 4 hour time delay between PER1 and PER2 oscillations. The expression rhythms appear to originate from retina.

Purification and Purity: affinity purified by Protein A

Specificity: PER2/Period circadian protein 2 Polyclonal Antibody detects endogenous levels of PER2/Period circadian protein 2 protein.

Bioworld Molecular Weight: ~138 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Paraformaldehyde-fixed, paraffin embedded (Mouse brain)Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PER2) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, Paraformaldehyde-fixed, paraffin embedded (Rat skeletal muscle)Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PER2) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C

Alternative Name: Circadian clock protein PERIOD 2; FASPS; hPER 2; hPER2; KIAA0347; PER 2; PER2; Period 2; Period 2 isoform 1; Period circadian protein 2; Period circadian protein homolog 2; Period homolog 2; Period2; FASPS; PER2_HUMAN.

Immunogen: KLH conjugated synthetic peptide derived from human PER2:751-850/1225

Modification: Unmodified

Conjugate: Unconjugated