Perforin 1 rabbit pAb - ES8510

Perforin 1 rabbit pAb

Sizes: 50μL, 100μL

Catalogue Numbers: ES8510-50, ES8510-100

Citations, Manuals and MSDS Available upon request.

Background: The protein encoded by this gene has structural and functional similarities to complement component 9 (C9). Like C9, this protein creates transmembrane tubules and is capable of lysing non-specifically a variety of target cells. This protein is one of the main cytolytic proteins of cytolytic granules, and it is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis. Defects in this gene cause familial hemophagocytic lymphohistiocytosis type 2 (HPLH2), a rare and lethal autosomal recessive disorder of early childhood. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jul 2008],

Alternate Name: Perforin-1 (P1) (Cytolysin) (Lymphocyte pore-forming protein) (PFP)

Source: Rabbit

Applications: WB; IHC; ELISA

Dilution: WB 1:500-2000, IHC 1:50-200, ELISA 1:10000-20000

Reactivity: Human; Rat; Mouse;

Immunogen: The antiserum was produced against synthesized peptide derived from the C-terminal region of human PRF1. AA range:451-500

Storage and Stability: -20°C/1 year

Clonality: Polyclonal

Isotype: IgG

Concentration: 1 mg/ml

Observed Band (KD): 61kD

Human Gene ID: 5551

Human SWISS Prot NO: P14222

Subcellular Location: Cytolytic granule. Secreted. Cell membrane; Multi-pass membrane protein. Endosome lumen. Stored in cytolytic granules of cytolytic T-lymphocytes and secreted into the cleft between T-lymphocyte and target cell (PubMed:20038786). Inserts into the cell membrane of target cells and forms pores (PubMed:20889983). Membrane insertion and pore formation requires a major conformation change (PubMed:20889983). May be taken up via endocytosis involving clathrin-coated vesicles and accumulate in a first time in large early endosomes (PubMed:20038786).

Research Use Only