Product Description

Perforin Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BZ16042-50, BZ16042-100

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Swiss-Prot: P14222

Host: Rabbit

Reactivity: Human

Applications: WB, IHC-P, ELISA

All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100 ELISA: 1/10000

Background: The protein encoded by this gene has structural and functional similarities to complement component 9 (C9). Like C9, this protein creates transmembrane tubules and is capable of lysing non-specifically a variety of target cells. This protein is one of the main cytolytic proteins of cytolytic granules, and it is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis. Defects in this gene cause familial hemophagocytic lymphohistiocytosis type 2 (HPLH2), a rare and lethal autosomal recessive disorder of early childhood. Alternative splicing results in multiple transcript variants encoding the same protein.

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IgG

Bioworld Molecular Weight: Calculated MW: 61 kDa; Observed MW: 61 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Perforin in Hela lysates using Perforin antibody., Immunohistochemistry analysis of paraffin-embedded Human kidney using Perforin antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval., Immunohistochemistry analysis of paraffin-embedded Human kidney using Perforin antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval., Immunohistochemistry analysis of paraffin-embedded Human spleen using Perforin antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Alternative Name: Perforin-1; P1; Cytolysin; Lymphocyte pore-forming protein; PFP

Immunogen: The antiserum was produced against synthesized peptide derived from the C-terminal region of human PRF1. AA range:451-500

Conjugate: Unconjugated

Modification: Unmodified