Product Description

PERK (phospho Thr981) Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES1527-50, ES1527-100

Lead times: approximately 7-10 business days, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The protein encoded by this gene phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2, leading to its inactivation, and thus to a rapid reduction of translational initiation and repression of global protein synthesis. This protein is thought to modulate mitochondrial function. It is a type I membrane protein located in the endoplasmic reticulum (ER), where it is induced by ER stress caused by malfolded proteins. Mutations in this gene are associated with Wolcott-Rallison syndrome. [provided by RefSeq, Sep 2015],

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: IF, WB, IHC, ELISA

Alternate Names: EIF2AK3; PEK; PERK; Eukaryotic translation initiation factor 2-alpha kinase 3; PRKR-like endoplasmic reticulum kinase; Pancreatic eIF2-alpha kinase; HsPEK

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from human PEK/PERK around the phosphorylation site of Thr981. AA range:947-996

Isotype: IgG

Target: PERK,

Specificity: Phospho-PERK (T981) Polyclonal Antibody detects endogenous levels of PERK protein only when phosphorylated at T981.

Dilution: IF: 1:50-200 WB 1:500-2000 , Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Ubiquitous. A high level expression is seen in secretory tissues.

Observed Band (KD): 130kD

Fields: Mitophagy - animal, Autophagy - animal, Protein processing in endoplasmic reticulum, Apoptosis, Non-alcoholic fatty liver disease, Alzheimer disease, Parkinson disease, Amyotrophic lateral sclerosis, Prion disease, Pathways of neurodegeneration - multiple diseases, Hepatitis C, Measles, Herpes simplex virus 1 infection, Lipid and atherosclerosis

Function: Catalytic Activity: ATP + a protein = ADP + a phosphoprotein. Disease: Defects in EIF2AK3 are the cause of Wolcott-Rallison syndrome (WRS) [MIM:226980]; also known as multiple epiphyseal dysplasia with early-onset diabetes mellitus. WRS is a rare autosomal recessive disorder, characterized by permanent neonatal or early infancy insulin-dependent diabetes and, at a later age, epiphyseal dysplasia, osteoporosis, growth retardation and other multisystem manifestations, such as hepatic and renal dysfunctions, mental retardation and cardiovascular abnormalities. Domain: The lumenal domain senses perturbations in protein folding in the ER, probably through reversible interaction with HSPA5/BIP. enzyme regulation:Perturbation in protein folding in the endoplasmic reticulum (ER) promotes reversible dissociation from HSPA5/BIP and oligomerization, resulting in transautophosphorylation and kinase act

Subcellular Location: Endoplasmic reticulum membrane, Single-pass type I membrane protein.

Storage and Stability: -20°C/1 year

Gene Name: EIF2AK3

Protein Name: Eukaryotic translation initiation factor 2-alpha kinase 3

Human Gene ID: 9451

Human SwissProt Number: Q9NZJ5

Mouse SwissProt Number: Q9Z2B5

Rat Gene ID: 29702

Rat SwissProt Number: Q9Z1Z1

Research Use Only