Product Description

PERK (R87) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS2156-50, BS2156-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: An interferon-inducible, RNA-dependent protein serine /threonine kinase (PKR) has been described. PKR in earlier literature is variously known as DAI, dsJ, PI kinase, p65, p67 or TIK for the mouse kinase, and p68 or p69 for the human kinase. The PKR kinase substrate is the α subunit of protein synthesis initiation factor eIF-2. Phosphorylation of eIF-2α on serine-51 resμlts in inhibition of translation. The serine /threonine kinase catalytic domains map to the carboxy terminal half of the protein while the RNA-binding domains are located in the amino terminal region. PERK is a type I transmembrane protein located in the endoplasmic reticμlum (ER) that contains a kinase domain similar to the kinase domain of PKR. PERK is activated in response to ER stress and phosphorylates eIF-2α, thus inhibiting the translation of mRNA.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC, IF

Alternate Names: Eukaryotic translation initiation factor 2-alpha kinase 3, PRKR-like endoplasmic reticμlum kinase, Pancreatic eIF2-alpha kinase, HsPEK, EIF2AK3, PEK, PERK

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Unmodified

Immunogen: Synthetic peptide, corresponding to amino acids 56-102 of Human PERK.

Dilution: WB: 1:500~1:1000
IHC: 1:50~1:200
IF: 1:50~1:200

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: PERK (R87) polyclonal antibody detects endogenous levels of PERK protein.

Molecular Weight: ~ 140 kDa

Pathway: Translational Contral Regμlation of elF2,

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: Q9NZJ5

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only