Peroxiredoxin 2 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS91055-50, BS91055-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P32119(Human) Q61171(Mouse) P35704(Rat)
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, ICC/IF, IHC, FC
All Applications: WB:1:1,000-1:2,000
ICC:1:100-1:500
IHC:1:50-1:200
FC:1:10-1:50
Background: The peroxiredoxin (PRX) family comprises six antioxidant proteins, PRX I, II, III, IV, V and VI, which protect cells from reactive oxygen species (ROS) by preventing the metal-catalyzed oxidation of enzymes. The PRX proteins primarily utilize thioredoxin as the electron donor for antioxidation, although they are fairly promiscuous with regard to the hydroperoxide substrate. In addition to protection from ROS, peroxiredoxins are also involved in cell proliferation, differentiation and gene expression. PRX I, II, IV and VI show diffuse cytoplasmic localization, while PRX III and V exhibit distinct mitochondrial localization. PRX II is expressed in testis, while PRX III shows expression in lung. PRX I, II and III are overexpressed in breast cancer and may be involved in its development or progression. Upregulated protein levels of PRX I and II in Alzheimer's disease (AD) and Down syndrome (DS) indicate the involvement of PRX I and II in their pathogenesis.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: Peroxiredoxin 2 polyclonal antibody detects endogenous levels of Peroxiredoxin 2 protein.
Bioworld Molecular Weight: 22 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of Peroxiredoxin 2 on different lysates using anti-Peroxiredoxin 2 antibody at 1/1,000 dilution.
Positive control:
Lane 1: Hela
Lane 2: HepG2, ICC staining Peroxiredoxin 2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: NKEF-B, TSA, PRDX2, Epididymis secretory sperm binding protein Li 2a, HEL S 2a, MGC4104, Natural killer cell enhancing factor B, Natural killer cell-enhancing factor B, Natural Killer Enhancing Factor B, NKEF B, NKEF-B, NKEFB, Peroxiredoxin-2, PRDX 2, PRDX2, PRDX2, PrP, PRX2, PRXII, PTX1, TDPX1, Thiol Specific Antioxidant 1, Thiol specific antioxidant protein, Thiol-specific antioxidant protein, Thioredoxin Dependent Peroxide Reductase 1, Thioredoxin peroxidase 1, Thioredoxin-dependent peroxide reductase 1, Torin, TPX1, TSA,
Immunogen: recombinant protein
Conjugate: Unconjugated
Modification: Unmodified