Product Description

PFDN6 Polyclonal Antibody

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Sizes: 50µl, 100µl

Catalogue Numbers: BS74562-50, BS74562-100

Swiss-Prot: O15212

Host: Rabbit

Reactivity: Mouse

Applications: WB

All Applications: WB,1:500 - 1:2000

Background: PFDN6 is a subunit of the heteromeric prefoldin complex that chaperones nascent actin (see MIM 102560) and alpha- and beta-tubulin (see MIM 602529 and MIM 191130, respectively) chains pending their transfer to the cytosolic chaperonin containing TCP1 (MIM 186980) (CCT) complex (Hansen et al., 1999 [PubMed 10209023]).[supplied by OMIM, Jul 2010]

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Unmodification

Bioworld Molecular Weight: 14kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of mouse liver, using PFDN6 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 30s.

Alternative Name: PFDN6; H2-KE2; HKE2; KE-2; PFD6

Immunogen: Recombinant fusion protein of human PFDN6 (NP_055075.1).

Conjugate: Unconjugated

Modification: Unmodified