Product Description

PFKFB1/4 (E349) Polyclonal Antibody

Catalogue Numbers: BS2079-50, BS2079-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P16118/Q16877

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: PFK-1 undergoes activation in the presence of elevated AMP. The most potent activator is fructose-2,6-bisphosphate, which is produced by PFK-2 from the same substrate, fructose 6-phosphate. PFK-2 is bifunctional and a key regulator for PFK-1. PFK-2 catalyzes the synthesis of fructose-2,6-bisphosphate, and contains fructose-2,6-biphosphatase activity that catalyzes the degradation of fructose-2,6-bisphosphate. PFK-2 is dimeric and isoenzymes include PFK-2 liver (PFKFB1, PFRX), PFK-2 cardiac (PFKFB2), PFK-2 placental (PFKFB3, inducible PFK-2) and PFK-2 testis (PFKFB4).

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: PFKFB1/4 (E349) polyclonal antibody detects endogenous levels of PFKFB1/4 protein.

Extra Notes: Western blot (WB) analysis of PFKFB1/4 (E349) pAb at 1:1000 dilution
Lane1:K562 whole cell lysate(40ug)
Lane2:A549 whole cell lysate(40ug)
Lane3:PC12 whole cell lysate(40ug)
Lane4:AML-12 whole cell lysate(40ug)

Bioworld Molecular Weight: ~ 54 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: PFK-2 liver; PFKFB1; PFRX; F6PK; 6PF-2-K/Fru-2; 6-P2ASE liver isozyme; 6-phosphofructo-2-kinase/fructose-2; 6-biphosphatase 1; PFK-2 testis; 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4; 6PF-2-K/Fru-2,6-P2ase 4; PFK/FBPase 4; 6PF-2-K/Fru-2,6-P2ase testis-type isozyme; 6-phosphofructo-2-kinase; Fructose-2,6-bisphosphatase; PFKFB4

Immunogen: Synthetic peptide, corresponding to amino acids 320-370 of Human PFKFB1.

Conjugate: Unconjugated

Modification: Unmodified