PFN4 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS75414-50, BS75414-100
Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q8NHR9
Host: Rabbit
Reactivity: Mouse, Rat
Applications: WB
All Applications: WB,1:500 - 1:2000
Background: Binds to phosphatidylinositol 3-phosphate (PtdIns(3P, phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5P2, phosphatidylinositol 4-phosphate (PtdIns(4P and phosphatidic acid (PA. Does not bind to actin, contrary to other family members.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: Unmodification
Extra Notes: Western blot analysis of extracts of various cell lines, using PFN4 antibody at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 5min.
Note: For research use only, not for use in diagnostic procedure.
Bioworld Molecular Weight: 14kDa
Alternative Name: PFN4
Immunogen: Recombinant fusion protein of human PFN4(NP_955378.1).
Conjugate: Unconjugated
Modification: Unmodified