PGHS-2 Monoclonal Antibody
Catalogue Numbers: MB66973-50, MB66973-100
Sizes: 50µl, 100µl
Product: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Swiss-Prot: P35354
Host: Mouse
Reactivity: Human
Applications: WB
All Applications: WB (1/500 - 1/1000)
Background: Cyclooxygenase1 (Cox1) and cyclooxygenase2 (Cox2), family members with 60% homology in humans, catalyze prostaglandin production from arachidonic acid . While Cox1 expression is constitutive in most tissues, Cox2 expression is induced by lipopolysaccharide (LPS) and peptidoglycan (PGN) . PGN activates Ras, leading to phosphorylation of Raf at Ser338 and Erk1/2 at Tyr204. The activation of MAP kinase signaling results in subsequent activation of IKKα/β, phosphorylation of IκBα at Ser32/36, and NF-κB activation. Finally, activation of the transcription factor NF-κB is responsible for the induction of Cox2 expression . Investigators have shown that LPS and PGN induce the clinical manifestations of arthritis and bacterial infections, such as inflammation, fever, and septic shock . Research studies have indicated that Cox1 and Cox2 may also play a role in the neuropathology of Alzheimer's disease by potentiating γ-secretase activity and β-amyloid generation .
Purification and Purity: This antibody is purified through a protein G column.
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: Recognizes endogenous levels of PGHS-2 protein.
Extra Notes: Western blot analysis of PGHS-2 expression in U87MG (A) whole cell lysates.
Bioworld Molecular Weight: ~ 74 kDa
Note: For research use only, not for use in diagnostic procedure.
Alternative Name: COX2; Prostaglandin G/H synthase 2; Cyclooxygenase-2; COX-2; PHS II; Prostaglandin H2 synthase 2; PGH synthase 2; PGHS-2; Prostaglandin-endoperoxide synthase 2
Immunogen: Recombinant fusion protein of human PGHS-2. The exact sequence is proprietary.
Conjugate: Unconjugated
Modification: Unmodified