Product Description

PGK1 Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Sizes: 50µl, 100µl

Catalogue Numbers: BS6691-50, BS6691-100

Swiss-Prot: P00558

Host: Rabbit

PGK1 Polyclonal Antibody-BS6691

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Reactivity: Human, Mouse

Applications: WB, IHC

All Applications: WB: 1:500~1:1000
IH: 1:50~1:200

Background: Phosphoglycerate kinases 1/2 (PGK1/2, ATP:3-phospho-D-glycerate 1-phosphotransferase, EC 2.7.2.3) are somatically expressed, glycolytic enzymes that catalyze the transfer of a phosphoryl group from the acyl phosphate of 1,3-bisphosphoglycerate to ADP, thereby forming ATP and 3-phosphoglycerate. The human PGK gene is interrupted by 10 introns and spans 23 kilobases, and is X chromosome-linked at position Xq21.1, a region implicated in prostate cancer, androgen insensitivity, perineal hypospadias, and other genetic abnormalities. In addition to influencing glycolysis, the PGK1 is secreted by tumor cells and contributes to proliferative angiogenic processes as a disulfide reductase. PGK1 mediated reduction of disulphide bonds in the serine proteinase plasmin initiates the release of the tumor blood vessel inhibitor angiostatin, an event that is critical for blood vessel formation or angiogenesis in tumor expansion and metastasis.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: PGK1 polyclonal antibody detects endogenous levels of PGK1 protein.

Bioworld Molecular Weight: ~ 44 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of PGK1 expression in Hela (A), PC3 (B), ES2 (C), mouse stomach (D) whole cellLysates., Immunohistochemical analysis of PGK1 staining in human colon formalin fixed paraffin embedded tissue section.

Alternative Name: Cell migration-inducing gene 10 protein; MGC117307; MGC8947; MIG10; pgk1; PGK1; PGKA; Phosphoglycerate kinase 1; Primer recognition protein 2; PRP 2; PRP 2;

Immunogen: Recombinant full length Human PGK1.

Modification: Unmodified

Conjugate: Unconjugated